Monoclonal Antibodies

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Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long or untagged Kv2.1 plasmids and probed with N320/48 (left) and Mouse anti-Flag (right).
Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N319A/14 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N309A/21 TC supe (left) and a rabbit polyclonal antibody control (right).
"Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N307/12 TC supe (left) and a rabbit polyclonal control (right). ."
Tissue and transfected cell immunoblot: extracts of rat eye and brain membrane (REM and RBM, respectively) and COS cells transiently transfected with Myc-tagged BBS5 or untagged Kv2.1 plasmid and probed with N304B/115 (left) and N52A/42 (right) TC supe.
Serial dilutions of BSA-conjugated target and non-target peptides dotted onto membrane and probed with N310/21 TC supe (left) and a rabbit polyclonal antibody control (right).
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N299/39 TC supe (left) and a mouse loading control (right).
Adult rat brain immunohistochemistry.Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged BBS3/Arl6 or untagged Kv2.1 plasmid and probed with N302/10 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult spinal cord sections from Brevican wild-type (WT) and knockout (KO) mice with N294A/10 (red) and Caspr rabbit polyclonal (green). Images courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine).
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N291C/22 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).Transfected cell immunoblot: extracts of COS cells transiently transfected with carboxy- terminally (C) and amino-terminally (N) GFP- tagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid and probed with N291C/22 (left) and N212/7 (right) TC supe.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N274/8 (left) and N86/8 (right).
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.
Immunoblot against bacterially expressed Thioredoxin (TRX) and/or 6xHistidine (His) tagged fusion proteins.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged TARPGamma2/3/4/8 or untagged Kv2.1 plasmid and probed with N245/36 (left) and N86/8 (right) TC supe.
Immunoblot versus low-speed pellet samples from wild-type (WT) and Stargazer mutant (STG) mice and probed with N245/1 TC supe. Data courtesy of Susumu Tomita, Yale University.
Adult rat brain membrane (RBM) immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Tissue and transfected cell immunoblot: low speed pellet samples from adult rat brain (RBLSP) and extracts of COS cells transiently transfected with Myc-tagged PEX6 or untagged Kv2.1 plasmids and probed with N233/8 (left) and K89/34 (right).
Adult rat hippocampus (top), thalamus (bottom left) and superior colliculus (bottom right) immunohistochemistry with antigen retrieval via pepsin pretreatment.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Adult rat brain immunohistochemistry.

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