Anti-Vimentin Antibody (V9)

Name: Anti-Vimentin Antibody
Brand: PhosphoSolutions
SKU: VM4341
Price: 292.0 USD
Availability: InStock

Our Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Vimentin and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of Vimentin in paraformaldehyde fixed and NP-40 permeabilized A7r5 cells. The cells were labeled with a mouse monoclonal antibody to Vimentin (VM4341), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate
filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).

Immunocytochemical labeling of Vimentin in paraformaldehyde fixed and NP-40 permeabilized A7r5 cells. The cells were labeled with a mouse monoclonal antibody to Vimentin (VM4341), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: VM4341

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Vimentin

Scientific Background

Intermediate filaments (IF) are important components of the cytoskeleton and nuclear envelope. IF protein family members are encoded by 70 genes that have diverse expression patterns during development and tissue-specific functions. For example, the keratins form IFs in epithelial cells, while vimentin forms filaments in mesenchymal, endothelial, and hematopoietic cells. Neurofilament triplet proteins and internexins form IFs in neurons, while desmin, synemin and syncoilin form the extra-sarcomeric cytoskeleton of myoblasts. The nuclear IF system consists of lamin family proteins organized in a meshwork-like lamina around the nucleus. Vimentin is a 58 kDa IF protein that forms 10 nm filaments that are important for mechanical stability, motility, and adhesion in various cell types. Vimentin activity is regulated by proteolysis and phosphorylation. Rho-kinases can phosphorylate vimentin leading to destabilization of intermediate filaments, while Akt phosphorylation of Ser-39 in vimentin reduces proteolysis of vimentin and promotes cancer cell motility and invasion.

Format

Protein A Purified

Clonality

Monoclonal

Clone

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

VIM

Molecular Weight

Immunogen

Clone (V9) was generated from full length vimentin purified from porcine eye lens. The antibody reacts with human, pig, chicken, rat, and mouse vimentins.

Immunogen Species

Pig

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:200
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 58 kDa* protein corresponding to the molecular mass of vimentin on SDS-PAGE immunoblots of human fibroblasts (HS-68). In immunocytochemistry, the antibody detects intermediate filaments in rat smooth muscle cells (A7r5).

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

VIM

UniProt ID

P02543

Shipping

Blue Ice

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Anti-Vimentin Antibody (V9)

SKU: VM4341

Product Details

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