Antibody-Sandwich ELISA Development Kit
Assess assay feasibility and optimize ELISA performance parameters with ICT’s Antibody-Sandwich ELISA Development Kit. The Antibody-Sandwich ELISA Development Kit provides eight specially formulated ELISA Solutions and a template for the initial development and optimization of antibody-sandwich ELISA tests. A comprehensive ELISA development manual is supplied with protocols for assessing initial assay feasibility and optimizing ELISA performance parameters, which will be beneficial to both novice or experienced users. From coating buffer to substrate stop solution, this kit contains enough reagent to construct ten 96-well ELISA plates.
Antibody-Sandwich ELISA Development Kit
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Ships: 1-2 business days
Milestones Common to All Sandwich ELISA Development Projects
- Acquisition of adequate quantities of quality antibody (IgG) and analyte components necessary to initiate project.
- Preparation or acquisition of the sandwich IgG-HRP conjugates.
- Demonstration of basic assay feasibility (dose response).
- Optimization of capture antibody coating.
- Resolution of sample matrix interference issues.
- Final optimization of sandwich IgG-HRP conjugate concentration, considering the inhibitory effects of the sample matrix.
- Demonstration of ELISA performance capabilities via assessment of assay linear dynamic range, sensitivity, linearity, standard recovery efficiency, and precision. For detailed protocols and information on each of these ELISA development milestones, please see the Antibody-Sandwich ELISA Development Kit Manual.