ECM Biosciences

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Fluorescent labeling of actin filaments in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with phalloidin:TRITC (PF7551) at a dilution of 1:2000.
Fluorescent labeling of actin filaments in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with phalloidin:FITC (PF7501) at a dilution of 1:500.
Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).
Western blot analysis of WWP1 expression in adult mouse liver (lane 1) and human prostate adenocarcinoma (PC3) cells (lane 2). The blot was probed with anti-WWP1 (WP3931) at 1:1000.
Western blot of rat PC12 lysate. The blots were probed with anti-WAVE2 (Central region) in the presence (lane 2) or absence (lane 1) of WAVE2 (Central region) blocking peptide.Immunocytochemical labeling of WAVE2 in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal WAVE2 (Central) antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (WX1795).

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