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Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
PhosphoSolutions Anti-Phosphoserine/threonine Antibody
$340
Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AP2041; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AP2041) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Atrogin-1 Antibody
$340
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Phosphoserine/threonine Antibody
$340
Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8).Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.
PhosphoSolutions Anti-Myosin Light Chain (Ser-19), Phosphospecific Antibody
$340
Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).Immunocytochemical labeling of MuRF1 in mouse C2C12 cells. The cells were labeled with rabbit polyclonal MuRF1 antibody, then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3405).
PhosphoSolutions Anti-MuRF1 (C-terminal region) Antibody
$340
Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WAVE1 (N-terminal region) peptide (lane 5) or WAVE2 (Central region) peptide (lane 6).Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-WAVE1 (N-terminal region) Antibody
$340
Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with rabbit polyclonal anti-TRPM8 (extracellular region) without peptide blocking (lane 1) or with peptide blocking (lane 2).Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with rabbit polyclonal anti-TRPM8 (TP5701). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-TRPM8 (Extracellular region) Antibody
$340
Western blot analysis of adult mouse diaphram treated with thapsigargin for 0, 6, 12, or 24 hours to induce cleavage of αII-spectrin from 250 kDa to 136 kDa. The blot was probed with rabbit polyclonal αII-spectrin (a.a. 1171-1176) cleavage-specific antibody at a dilution of 1:1,000 (Image provided by Dr. Leigh Ann Callahan, Department of Internal Medicine, University of Kentucky.)
PhosphoSolutions Anti-αII-Spectrin , cleavage-specific Antibody
$340
Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-Myosin IIA Heavy Chain (Ser-1916), Phosphospecific Antibody
$340
Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3795).
PhosphoSolutions Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody
$285
Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).
PhosphoSolutions Anti-Histone H2B (N-terminal region) Antibody
$340
Paraformaldehyde-fixed zebrafish embryos were probed with anti-EphA4 (Tyr-602) (EP2731) then detected using Alexa Fluor 647 goat anti-rabbit. Arrows show labeling of somite boundaries and the notochord. (Image provided by Dr. Scott Holley at the Department of Molecular, Cellular and Developmental Biology, Yale University.)Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).
PhosphoSolutions Anti-EphA4 (Tyr-602) [conserved site], Phosphospecific Antibody
$340
Recombinant human eEF2K untreated (lanes 1 and 4) and phosphorylated with p38 kinase in vitro (lanes 2 & 5). After in vitro reaction, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-359) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.)
PhosphoSolutions Anti-eEF2K (Ser-359), Phosphospecific Antibody
$340
Western blot analysis of anti-γ-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with γ-Catenin (a.a. 545-555), γ-Catenin (Tyr-550) or γ-Catenin (C-terminal) antibodies. The anti-γ-Catenin (Tyr-550) was used in the presence of γ-Catenin (Tyr-550) (lane 5) or γ-Catenin (Tyr-644) (lane 6) peptides.Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal γ-Catenin (CM1111) or rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.
PhosphoSolutions Anti-γ-Catenin (Tyr-550), Phosphospecific Antibody
$340
Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University)
PhosphoSolutions Anti-Asc (Tyr-144), Phosphospecific Antibody
$340
PhosphoSolutions Sema-3E and Plexin D1 Receptor Antibody Kit
$459
PhosphoSolutions Slingshot-1L Phospho-Regulation Antibody Kit
$459
PhosphoSolutions c-Src Phospho-Regulation Antibody Kit
$459
PhosphoSolutions SHP1 Phospho-Regulation Antibody Kit
$459
PhosphoSolutions Sema-4D and Plexin B1 Receptor Antibody Kit
$459
PhosphoSolutions Sema-3A and NRP1/Plexin A1 Receptor Antibody Kit
$459
PhosphoSolutions Stat Phospho-Regulation Antibody Kit
$459
PhosphoSolutions Sphingosine Kinase 1 Phospho-Regulation Antibody Kit
$459
PhosphoSolutions Robo1 & Robo2 Antibody Kit
$459

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