ECM Biosciences

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Western blot image of human PC3 cells untreated (lanes 1 & 3) or treated with alkaline phosphatase to dephosphorylate histone H4 (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-Histone H4 (lanes 1 & 2) and anti-Histone H4 (Tyr-72) phospho-specific antibody (lanes 3 & 4).
PhosphoSolutions Anti-Histone H4 (Tyr-72), Phosphospecific Antibody
$340
Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).
PhosphoSolutions Anti-Histone H2B (Ser-36), Phosphospecific Antibody
$340
Western blot of human recombinant DDR1 protein with C-terminal His Tag. The blot was probed with mouse monoclonal anti-His (Cterminal) Tag (HM0501) at 1:2000 (lane 1), 1:4000 (lane 2), and 1:8000 (lane 3).Immunocytochemical labeling of Axl recombinant protein with C-terminal His Tag bound to THP1 aldehyde fixed cells. The cells were labeled with mouse monoclonal anti-His (C-terminal) Tag antibody (HM0501). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-His (C-terminal) Tag Antibody
$285
Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3β (lanes 1 & 2) or anti-GSK-3α/β (Y279/Y216) (lanes 3 & 4).
PhosphoSolutions Anti-GSK-3α/β (Tyr-279/Tyr-216), Phosphospecific Antibody
$340
Western blot analysis of a human recombinant GSK3β N-terminal fragment (lanes 1 & 2) and endogenous GSK3β expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3β at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).Immunocytochemical labeling of GSK3β in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK3β (GM1311) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-GSK-3β (N-terminal region) Antibody
$285
Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).Immunocytochemical labeling of Grb2 in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Grb2 (GM3311) antibody, then detected using appropriate secondary antibody conjugated to Cy3.
PhosphoSolutions Anti-Grb2 Antibody
$370
Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.
PhosphoSolutions Anti-GM130 (C-terminal region) Antibody
$370
Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).
PhosphoSolutions Anti-Girdin (Tyr-1764), Phosphospecific Antibody
$340
Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1674), phospho-specific (lanes 3 & 4).
PhosphoSolutions Anti-Girdin (Ser-1674), Phosphospecific Antibody
$340
Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1416), phospho-specific (lanes 3 & 4).
PhosphoSolutions Anti-Girdin (Ser-1416), Phosphospecific Antibody
$340
Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Girdin (C-terminus) Antibody
$340
Immunocytochemical labeling of FXYD5 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal FXYD5 (FM0311). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-FXYD5/Dysadherin (Extracellular region) Antibody
$340
Western blot analysis of FXYD5 protein expression. Immunoprecipitates using FM0311 FXYD5 antibody only (lane 1) or FM0311 with A431 lysate (lane 2) or A431 input only (lane 3). Human cell lysates MDA-MB-231 (lane 4) or MeWo (lane 5). The blot was probed with mouse monoclonal FXYD5 (FM0291) at 1:1000.Immunocytochemical labeling of FXYD5 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal FXYD5 (FM0291). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-FXYD5/Dysadherin (Extracellular region) Antibody
$370
Western blot image of human plasma purified fibronectin (lane 1), human breast tissue (lane 2), human A549 cells (lane 3), and LNCaP cells (lane 4). The blot was probed with mouse monoclonal anti-fibronectin FM0221 at 1:500.Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M022). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Fibronectin Antibody
$370
Western blot image of native human cell lysates: A549 (lanes 1 & 5), MeWo (lanes 2 & 6), and MDA-MB-231 (lanes 3 & 7), as well as human plasma fibronectin (lanes 4 & 8). The blot was probed with mouse monoclonal antibodies anti-fibronectin FM0141 (lanes 1-4) or anti-fibronectin FM0131 (lanes 5-8) at 1:1000.Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M013). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Fibronectin (Cell/Heparin2 Binding region) Antibody
$370
Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse polyclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .
PhosphoSolutions Anti-FHOD1 (Thr-1141), Phosphospecific Antibody
$340
Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .
PhosphoSolutions Anti-FHOD1 Antibody
$285
Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).Immunocytochemical labeling of fascin phosphorylation relative to F-actin in chick E9 DRG neurons. The cells were labeled with rabbit polyclonal Fascin (Ser-39) antibody, then detected using appropriate secondary antibody (Red). Fascin (Ser-39) labeling is compared (Top) to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).
PhosphoSolutions Anti-Fascin (Ser-39), Phosphospecific Antibody
$340
Western blot analysis of mouse C2C12 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) mouse monoclonal (lanes 3 & 4).
PhosphoSolutions Anti-Fascin (Ser-39), Phosphospecific Antibody
$340
Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).Immunocytochemical labeling of Fascin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Fascin (FM2651). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Fascin (clone 55K2) Antibody
$285
Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).
PhosphoSolutions Anti-FAK (Tyr-397), Phosphospecific Antibody
$340
Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-Estrogen Receptor α (Tyr-537), Phosphospecific Antibody
$340
Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-Estrogen Receptor α (Tyr-537), Phosphospecific Antibody
$340
Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).Immunocytochemical labeling of Estrogen Receptor α in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor α (EP5431). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-Estrogen Receptor α (C-terminus) Antibody
$285

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