Anti-E-Cadherin (Cytoplasmic) Recombinant Chicken Chimeric mAb FL594 Conjugate (M168)
Our chicken Anti-E-Cadherin (Cytoplasmic) FL594 conjugate recombinant monoclonal antibody is a chimeric antibody derived from clone M168. It detects human, mouse, and rat E-Cadherin (Cytoplasmic) and is purified by affinity chromatography. Works in ICC, IHC.
Anti-E-Cadherin (Cytoplasmic) Recombinant Chicken Chimeric mAb FL594 Conjugate
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
Purified by affinity chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Recombinant
M168
IgY
ICC, IHC
Chicken
CDH1
120 kDa
FL594 Ex: 594 nm, Em: 615 nm
Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin.
Mouse
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone M168 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone M168 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
IHC: 1:250-1:500
ICC: 1:250-1:1000
594
Cross-reacts with GFAP-R416W and other GFAP mutant proteins. Does not cross-react with other proteins (based on KO validation results).
Each new lot of antibody is quality control tested by Western blot on MCF-7 cell lysates and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
