Methyl Green
This methyl green counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology.
Methyl Green
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Ships: 1-2 business days
A. Immunohistochemistry:
- After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di water
- Apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes).
- DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X.
- Clear with Xylene or clearing agent.
- The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent.
- Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections.
- At the counterstain step, apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes).
- DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X.
- Clear with Xylene or clearing agent.
- The slides can be mounted with Organic mounting mediums like Organo mount (ImmunoBioscience cat. # AR-6504) or equivalent.