BCIP/NBT
Our unique BCIP/NBT is an excellent one component chromogen substrate system that is used in Immunohistochemistry (IHC), Immunocytochemistry (ICC), western blot (WB) and in situ hybridization with alkaline phosphatase
Our unique BCIP/NBT is an excellent one component chromogen substrate system that is used in Immunohistochemistry (IHC), Immunocytochemistry (ICC), western blot (WB) and in situ hybridization with alkaline phosphatase (alk phos). Alkaline phosphatase catalyzes the hydrolysis of a variety of phosphate containing substances in the alkaline pH range. This chromogen system gives purple blue color; is compatible with nuclear fast red (AR-6524) counterstain and can be mounted with aqueous mounting medium or our ImmunoHistoMount (AR-6503) This chromogen can also be counterstained with our Red counter stain (AR-6523) and mounted with mounting medium Immuno In Situ Mount (AR-6507)
2-8 °C
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
Application: IHC, ICC and in situ hybridization
- After the alkaline phosphatase reaction, wash slide with high pH buffer (~Tris/HCl 9.5).
- Add few drops of ready to use BCIP/NBT reagent, incubate at RT or 37ºC for 5-15 minutes.
- Wash 2-5X with buffer, followed by rinsing with distilled/deionized water.
- Add counterstain compatible with this chromogen (see description above).
- Wash with tap water, followed by rinsing with distilled/deionized water.
- Mount with aqueous mounting medium (see description above).