Anti-Pro-glial cell line-derived neurotrophic factor (proGDNF) Antibody (BS376)

Our Anti-Pro-glial cell line-derived neurotrophic factor (proGDNF) mouse monoclonal primary antibody detects human, mouse (predicted), and rat Pro-glial cell line-derived neurotrophic factor (proGDNF), and is IgG. It is validated for use in FC, ICC.

Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to proGDNF (cat # M-1727-100, 2 μg per ~106 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of proGDNF expressed in human neuronal cell line SHSY-5Y using cat # M-1727-100.
Immunofluorescence analysis of proGDNF expression in human SH-SY5Y cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) SH-SY5Y cells were incubated with proGDNF antibody M-1727-100 (2 µg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 µg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). proGDNF-IR is observed throughout the cytoplasm. Magnification: 100x.
Immunofluorescence analysis of proGDNF expression in rat C6 glioma cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) C6 cells were incubated with proGDNF antibody M-1727-100 (2 µg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 µg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). proNGF-IR is observed in the perinuclear cytoplasm. Magnification: 100x.
Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to proGDNF (cat # M-1727-100, 2 μg per ~106 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of proGDNF expressed in human neuronal cell line SHSY-5Y using cat # M-1727-100.
Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to proGDNF (cat # M-1727-100, 2 μg per ~106 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of proGDNF expressed in human neuronal cell line SHSY-5Y using cat # M-1727-100.
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Human, Mouse, Rat
Flow, ICC
Mouse

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Data Sheet

SKU: M-1727-100

Volume/Size: 100 µg
Price:
$327.00

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