Anti-Parkinson disease protein 7 (PARK7) Antibody (4H4)

Name: Anti-Parkinson disease protein 7 (PARK7) Antibody
Brand: Biosensis
SKU: M-1573-100
Price: 327.0 USD
Availability: InStock

Our Anti-Parkinson disease protein 7 (PARK7) mouse monoclonal primary antibody detects human Parkinson disease protein 7 (PARK7), and is IgG. It is validated for use in ICC, WB.

HeLa cells stained with Mouse monoclonal antibody to DJ-1 M-1573-100 (green), and Chicken polyclonal antibody to Vimentin C-1409-50 (red) and DNA (blue). M-1573-100 antibody reveals strong cytoplasmic staining for DJ-1.

Whole HeLa cell lysate stained with mouse anti-DJ1 antibody. The antibody recognizes the ~21 kDa protein.

Western blot analysis of whole brain and cell lysates using mouse antibody to DJ1 (green, 1:5,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The antibody detects DJ1 protein with apparent molecular weight of 21 kDa, but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken antibody to vimentin (C-1409-50, red, 1:5,000), revealing a single band at about 50 kDa (lanes 2-7).

HeLa cells stained with Mouse monoclonal antibody to DJ-1 M-1573-100 (green), and Chicken polyclonal antibody to Vimentin C-1409-50 (red) and DNA (blue). M-1573-100 antibody reveals strong cytoplasmic staining for DJ-1.

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Species Reactivity

Human

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1573-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Parkinson disease protein 7 (PARK7)

Scientific Background

Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinson's disease.

Format

IgG

Clonality

Monoclonal

Clone

4H4

Applications

ICC, WB

Host Species

Mouse

Molecular Weight

21 kDa

Immunogen

Full length recombinant human DJ-1 expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:5000
ICC: 1:500

Application Details

This antibody reveals a prominent ~21 kDa band and stains mainly in cytoplasm of tissue culture cells.

Conjugate

Unconjugated

Specificity

The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry. Does not react with rat and mouse DJ-1 protein on Western blots.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Oncogene DJ1; Parkinson disease protein 7; PARK7; DJ-1

UniProt ID

Q99497

Shipping

25°C (ambient)

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Anti-Parkinson disease protein 7 (PARK7) Antibody (4H4)

SKU: M-1573-100

Product Details

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