Anti-Galectin-3 (Gal-3) Antibody (5C21)
Our Anti-Galectin-3 (Gal-3) mouse monoclonal primary antibody detects human, mouse, other mammals (predicted), and rat Galectin-3 (Gal-3), and is affinity-purified. It is validated for use in ICC, WB.
![Left: Analysis of galectin-3 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with mouse antibody to galectin-3 (red, 1:1,000), and co-stained with chicken antibody to MAP2 (C-1382-50, green, 1:10,000). Blue: Hoechst nuclear stain. The galectin-3 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while MAP2-antibody labels dendrites and perikarya of mature neurons. Right: Western blot analysis of tissue and cell lysates using mouse antibody to galectin-3 (green, 1:2,000): [1] protein standard, [2] mouse brain lysate; [3] - [7] rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at 30 kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with an antibody to HSP60 (red, lanes 2-8).](http://www.antibodiesinc.com/cdn/shop/files/m-1574-100-ihc-wb_120x120.jpg?v=1759273275)
Image shows rat brain neural cultures stained with Mouse Monoclonal Antibody to Galectin-3 M-1574-100 (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.
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