Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody

Our Anti-Neurofilament heavy polypeptide, phosphorylated (pNF-H) chicken polyclonal primary antibody detects bovine, dog, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament heavy polypeptide, phosphorylated (pNF-H), and is IgY preparation. It is validated for use in ELISA, IF, ICC, IHC, WB.

Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1386-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Chicken pAb, (red, 1:5,000) and co-stained with product R-1374-50, Glial Fibrillary Acidic Protein (GFAP), Rabbit pAb (green). The blue is DAPI staining of nuclear DNA. Method: following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free floating sections were stained. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes.
Analysis by western blot of pNF-H expression in spinal cord lysates with C-1386-50, (green, 1:20,000) with strong bands at approx 200-220kDa corresponding to the phosphorlylated form of NF-H. Lane 1: MWM; Lane 2: rat; Lane 3: mouse; Lane 4: cow. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.
Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1386-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Chicken pAb, (red, 1:5,000) and co-stained with product R-1374-50, Glial Fibrillary Acidic Protein (GFAP), Rabbit pAb (green). The blue is DAPI staining of nuclear DNA. Method: following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free floating sections were stained. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes.
Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1386-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Chicken pAb, (red, 1:5,000) and co-stained with product R-1374-50, Glial Fibrillary Acidic Protein (GFAP), Rabbit pAb (green). The blue is DAPI staining of nuclear DNA. Method: following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free floating sections were stained. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes.
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Bovine, Canine, Horse, Human, Mouse, Pig, Rat
ELISA, ICC, IF, IHC, WB
Chicken

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Data Sheet

SKU: C-1386-50

Volume/Size: 50 µL
Price:
$327.00

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