Anti-Microtubule-associated protein 2 (MAP2) Antibody

Name: Anti-Microtubule-associated protein 2 (MAP2) Antibody
Brand: Biosensis
SKU: C-1382-50
Price: 327.0 USD
Availability: InStock

Our Anti-Microtubule-associated protein 2 (MAP2) chicken polyclonal primary antibody detects bovine, human, mouse, and rat Microtubule-associated protein 2 (MAP2), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (red). The perikarya and dendrites of neurons are strongly and specifically stained with this antibody while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.

Left: Analysis of MAP2 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken anti-MAP2 (1:10,000, red), co-stained with a mouse antibody to MAP-tau (green). Blue: DAPI nuclear stain. The MAP2 antibody stains dendrites and perikarya of neurons, while the MAP-tau antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Right: Western blot analysis of whole brain tissue lysates using chicken antibody to MAP2 (1:50,000). [1] protein standard, [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. A strong band at around 280 kDa corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at lower dilutions.

View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Microtubule Associated Protein 2 C-1382-50 (red). The perikarya and dendrites of neurons are strongly and specifically stained with this antibody while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative. Cell nuclei are visualized with DAPI DNA stain.

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Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1382-50

Volume/Size: 50 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Microtubule-associated protein 2 (MAP2)

Scientific Background

Microtubules are 25 nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.

Format

IgY preparation

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Immunogen

Bovine MAP2 isolated from brain by the GTP microtubule cycling method.

Immunogen Species

Bovine

Species Reactivity

Bovine, Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:5000-1:10000
ICC: 1:5000-1:10000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by IC. Hu, rat, Ms, Bov.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Microtubule-associated protein 2; MAP-2; MAP2;

UniProt ID

P11137

Shipping

25°C (ambient)

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Anti-Microtubule-associated protein 2 (MAP2) Antibody

SKU: C-1382-50

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