Anti-Heat Shock Protein 27 (HSP-27) Antibody (6H11)

Name: Anti-Heat Shock Protein 27 (HSP-27) Antibody
Brand: Biosensis
SKU: M-1692-100
Price: 327.0 USD
Availability: InStock

Our Anti-Heat shock protein 27 (HSP-27) mouse monoclonal primary antibody detects human Heat shock protein 27 (HSP-27), and is IgG. It is validated for use in ICC, WB.

Crude extract of HeLa cells. The antibody recognizes the 27 kDa protein.

HeLa cells stained with M-1692-100 (red) and also with our chicken polyclonal antibody to Vimentin, (C-1409-50). The HSP27 antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins. The vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells.

Left: Analysis of HSP27 expression (green) in HeLa cells by Immunocytochemistry. HSP27 was stained with mouse antibody at 1:2,000 dilution, and cells were co-stained with a chicken antibody to HSP60 (red). Blue: DAPI nuclear stain. The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria. Right: Western blot analysis of HSP27 expression (green) in tissue or whole cell lysates using mouse anti-HSP27 antibody at 1:10,000 dilution. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa. The strong single band at 27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific. The blot was simultaneously probed with a rabbit antibody to HSP60 (red, lanes 2-6).

Crude extract of HeLa cells. The antibody recognizes the 27 kDa protein.

Click on image to zoom

Species Reactivity

Human

Applications

ICC, WB

Host Species

Mouse

Go to Full Product Details

Data Sheet

SKU: M-1692-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Heat shock protein 27 (HSP-27)

Scientific Background

The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an apparent molecular weight of 27 kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments.

Format

IgG

Clonality

Monoclonal

Clone

6H11

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Molecular Weight

27 kDa

Immunogen

Recombinant full length human HSP27 expressed in and purified from E. coli

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 5% trehalose, and sodium azide

Dilution Ranges

WB: 1:5000-1:10000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The antibody reacts with a 27 kDa band by Western blot on a crude extract from HeLa cells. It has also been used successfully for immunocytochemistry. Does not react with rodent protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

UniProt ID

P04792

Shipping

25°C (ambient)

Recover password

Create my account

Anti-Heat Shock Protein 27 (HSP-27) Antibody (6H11)

SKU: M-1692-100

Product Details

Bulk Order Anti-Heat Shock Protein 27 (HSP-27) Antibody

Related Products

Recently Viewed