Anti-Ghrelin Antibody

Name: Anti-Ghrelin Antibody
Brand: Biosensis
SKU: GP-032-50
Price: 327.0 USD
Availability: InStock

Our Anti-Ghrelin guinea pig polyclonal primary antibody detects human and sheep Ghrelin, and is whole serum. It is validated for use in IHC-Frozen.

Paraffin-embedded sheep abomasum was incubated in guinea pig polyclonal antibodies to the human ghrelin protein at the dilution of 1:1000 overnight followed by incubation with biotinylated secondary antibodies. Cell bodies and nerve terminals in the sheep abomasum are intensely stained. This figure shows staining of cells when no pre-absorption is performed.

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Species Reactivity

Human, Sheep

Applications

IHC

Host Species

Guinea Pig

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Data Sheet

SKU: GP-032-50

Volume/Size: 50 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Ghrelin

Scientific Background

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC

Host Species

Guinea Pig

Immunogen

A synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response.

Immunogen Species

Human

Species Reactivity

Human, Sheep

Storage

Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C or lower. After reconstitution, divide into aliquots and store at -20°C, or lower, for optimal long term stability. It is recommended that a reconstituted aliquot is stored at 2-8°C for no longer than 2 weeks. Allocation of an appropriate anti-bacterial agent can increase shelf life by several weeks. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.

Physical State

Lyophilized

Production Notes

Neat serum.

Dilution Ranges

IHC: 1:3000

Application Details

IHC performed in sheep abomasum demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of human ghrelin.

Conjugate

Unconjugated

Specificity

Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep and human. Other species have not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (lyophilized, unopened vial).

Synonyms

Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; M46 protein

UniProt ID

Q9UBU3

Shipping

25°C (ambient)

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Anti-Ghrelin Antibody

SKU: GP-032-50

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