Our mouse SCNN1G/Gamma-Subunit Epithelial Sodium Channel monoclonal antibody from Antibodies Incorporated is produced in-house from clone 11-35-1. It detects human SCNN1G/Gamma-Subunit Epithelial Sodium Channel and is Protein A or Protein G purified. Works in ELISA, IF, IHC, WB.
Immunohistochemistry detection of SCNN1G/Gamma-Subunit Epithelial Sodium Channel in human kidney tissue using Anti-SCNN1G/Gamma-Subunit Epithelial Sodium Channel Antibody (Cat. CAM 035-01-010). Tissue was mounted and pariffin fixed. Heat induced antigen retrieval was performed using citrate buffer, heated, and then blocked. After washing, the primary antibody (Cat. CAM 035-01-010) was incubated with the sample. Courtesy of Per Svenningsen, PhD.
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit. In the y-subunit, putative cleavage sites for prostasin, kallikrein (similar site as prostasin), elastase, and plasmin exist. Prostasin is a glycosylphosphatidylinositol anchored apical serine protease expressed in the collecting duct that activates yENaC at a site close to but in the C-terminal direction to the furin cleavage with putative release of a 43-residue inhibitory tract. (1)
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
11-35-1
IgG1/k
ELISA, IF, IHC, WB
Mouse
SCNN1G
74 kDa
Synthetic peptide seguence: VGGSIIHKAC
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
CAM 035-01 was used in ELISA.
Unconjugated
CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human yENaC and recognizes the C-terminal fragment starting from amino acid residue 182.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
