Our mouse Ghrelin monoclonal antibody from Antibodies Incorporated is produced in-house from clone 45. It detects human and rat Ghrelin and is Protein A or Protein G purified. Works in ELISA, WB.
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
45
IgG1/l
ELISA, WB
Mouse
GHRL
13 kDa (pro-ghrelin), 6 kDa (mature ghrelin)
Synthetic human Ser3-octanoyl ghrelin-28
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
ABS 050-45 binds human ghrelin in solution. ABS 050-45 (as a capture antibody) forms a pair with ABS 052-121 as a biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA.
Unconjugated
ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
