Our mouse Ghrelin monoclonal antibody from Antibodies Incorporated is produced in-house from clone 121. It detects canine, feline, human, mouse, and rat Ghrelin and is Protein A or Protein G purified. Works in ELISA, WB.
Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food reflect those of active ghrelin.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
121
IgG2b/k
ELISA, WB
Mouse
GHRL
13 kDa (pro-ghrelin), 6 kDa (mature ghrelin)
Synthetic ghrelin (14-28)
Human
Canine, Feline, Human, Mouse
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
ABS 052-121 binds human ghrelin in solution. ABS 052-121 (as biotinylated detection antibody) forms an antibody pair with ABS 050-45 (as capture antibody) for measuring human ghrelin in sandwich ELISA.
Unconjugated
ABS 052-121 binds human ghrelin.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
