Anti-Visinin-Like Protein 1 (VSNL1) Antibody (2D11)

Name: Anti-Visinin-Like Protein 1 (VSNL1) Antibody
Brand: PhosphoSolutions
SKU: 2145-VSNL1
Price: 456.0 USD
Availability: InStock

Our Anti-Visinin-Like Protein 1 (VSNL1) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Visinin-Like Protein 1 (VSNL1) and is Protein G purified. It is great for use in WB, IHC, ICC.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~22 kDa VSNL1 protein.

Immunofluorescence of a section of rat cerebellum labeled with anti-visinin-like 1 protein (Cat. 2145-VSNL1, 1:500, red) and anti-GFAP (Cat. 620-GFAP, green, 1:5000). The anti-VSNL1 revels protein expressed in the granule cell menbranes and in synapses in the white matter and molecular layers. The anti-GFAP reveals protein expressed in the processes of Bergmann glia and astroglia.

Immunofluorescence of rat cerebellum showing strong synaptic staining of VSNL1 (Cat. : 2145-VSNL1, green) in the molecular layer (ML) and MAP2 (Cat. 1100-MAP2, red, 1:2500). The blue stain is nuclear DNA.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~22 kDa VSNL1 protein.

Click on image to zoom

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 2145-VSNL1

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Visinin-Like Protein 1 (VSNL1)

Scientific Background

Visinin-like protein 1 (VSNL1), also known as VILIP1, is a calcium sensor protein expressed exclusively in neurons. Highest levels of VSNL1 expression are found in cerebellar Purkinje cells. VSNL1 has been implicated in the modulation of cell signaling cascades via regulation of adenyl cyclase activity (Braunewell et al., 1997). Additionally, VSNL1 has been associated with amyloid plaques and neurofibrillar tangles in Alzheimer’s disease (Schnurra et al., 2001).

Format

Protein G Purified

Clonality

Monoclonal

Clone

2D11

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

VSNL1

Molecular Weight

22 kDa

Immunogen

Recombinant human VSNL1 purified from E. coli.

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2492285

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:2000
WB Brain: 1:2000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~22 kDa VSNL1 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

21 kDa CABP; Hippocalcin like protein 3; Hippocalcin-like protein 3; HLP 3; HLP3; HPCAL 3; HPCAL3; HUVISL1; Neural visinin-like protein 1; Neural visinin-like type 1 protein; Neurocalcin alpha; NVL 1; NVL1; Nvp1; OZ1; Ratnvp1; VILIP; VILIP1; VILIP-1; Visinin; Visinin like 1; Visinin like protein 1; Visinin-like protein 1; VISL 1; VISL1; VISL1_HUMAN; VLP-1; Vnsl1; Vsnl1

UniProt ID

P62760
UniProt Summary: Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. {ECO:0000250}.

NCBI Gene ID

7447

Shipping

Blue Ice

VSNL1 Free Floating IHF Protocol: Download

VSNL1 Slide Mount IHF Protocol: Download

Western Blot Protocol: Download

General References

Braunewell, K.H., Spilker, C., Behnisch, T. and Gundelfinger, E.D., 1997. The Neuronal Calcium‐Sensor Protein VILIP Modulates Cyclic AMP Accumulation in Stably Transfected C6 Glioma Cells: Amino‐Terminal Myristoylation Determines Functional Activity. Journal of Neurochemistry, 68(5), pp.2129-2139. PMID: 9109541
Schnurra, I., Bernstein, H.G., Riederer, P. and Braunewell, K.H., 2001. The neuronal calcium sensor protein VILIP-1 is associated with amyloid plaques and extracellular tangles in Alzheimer's disease and promotes cell death and tau phosphorylation in vitro: a link between calcium sensors and Alzheimer's disease? Neurobiology of Disease, 8(5), pp.900-909. PMID: 11592857

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Anti-Visinin-Like Protein 1 (VSNL1) Antibody (2D11)

SKU: 2145-VSNL1

Product Details

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