Anti-VGAT Antibody (L118/80)

Name: Anti-VGAT Antibody
Brand: NeuroMab
SKU: 75-457
Price: 337.0 USD
Availability: InStock

Our mouse VGAT monoclonal antibody from NeuroMab is produced in-house from clone L118/80. It detects mouse and rat VGAT and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat brain striatum and globus pallidus immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

Adult rat superior colliculus immunohistochemistry.

Adult rat brain striatum and globus pallidus immunohistochemistry.

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Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-457

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VGAT

Scientific Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L118/80

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc32a1 Vgat Viaat

Molecular Weight

50 kDa

Immunogen

Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2651169

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

bA122O1.1; Vesicular inhibitory amino acid transporter; GABA and glycine transporter; GABA and glycine transporter; hVIAAT; Solute carrier family 32 member 1; SLC32A1; Vesicular GABA transporter; SLC32A 1; mVGAT; mVIAAT; solute carrier family 32 (GABA vesicular transporter member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN

UniProt ID

O35633
UniProt Summary: Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake.

NCBI Gene ID

22348

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Anti-VGAT Antibody (L118/80)

SKU: 75-457

Product Details

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