Anti-Vesicular GABA transporter (VGAT) Antibody

Name: Anti-Vesicular GABA transporter (VGAT) Antibody
Brand: PhosphoSolutions
SKU: 2100-VGAT
Price: 456.0 USD
Availability: InStock

Our Anti-Vesicular GABA transporter (VGAT) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, mouse, rat, and Xenopus Vesicular GABA transporter (VGAT) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Western blot of rat olfactory bulb lysate showing specific immunolabeling of the ~53 kDa VGAT protein.

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Species Reactivity

Chicken, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 2100-VGAT

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Vesicular GABA transporter (VGAT)

Scientific Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

SLC32A1

Molecular Weight

53 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat VGAT, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Chicken, Mouse, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate

Antibody Registry ID

AB_2492282

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification on a column made with the peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~53 kDa VGAT protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

bA122O1.1; GABA and glycine transporter; GABA vesicular transporter member 1; hVIAAT; SLC32A1; SLC32A 1; solute carrier family 32 (GABA vesicular transporter member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN; solute carrier family 32

UniProt ID

O35458
UniProt Summary: Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient (By similarity). May also transport beta-alanine (By similarity). Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (By similarity). {ECO:0000250|UniProtKB:O35458, ECO:0000250|UniProtKB:O35633}.

NCBI Gene ID

83612

Shipping

Blue Ice

VGAT IHF Protocol: Download

Western Blot Protocol: Download

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Anti-Vesicular GABA transporter (VGAT) Antibody

SKU: 2100-VGAT

Product Details

Bulk Order Anti-Vesicular GABA transporter (VGAT) Antibody

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