Anti-Tyrosine Hydroxylase (Ser40) Antibody

Name: Anti-Tyrosine Hydroxylase (Ser40) Antibody
Brand: PhosphoSolutions
SKU: p1580-40
Price: 456.0 USD
Availability: InStock

Our Anti-Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects many mammalian, and many non-mammalian Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.

Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.

Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.

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Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p1580-40

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Tyrosine Hydroxylase (Ser40)

Scientific Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

Molecular Weight

60 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Antibody Registry ID

AB_2492279

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:50-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over non-phospho TH.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser40

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_HUMAN; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; Tyrosine hydroxylase

UniProt ID

P04177
UniProt Summary: Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:15287903, PubMed:1680128, PubMed:17391063, PubMed:24753243, PubMed:34922205, PubMed:8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). {ECO:0000250|UniProtKB:P04177, ECO:0000250|UniProtKB:P24529, ECO:0000269|PubMed:15287903, ECO:0000269|PubMed:1680128, ECO:0000269|PubMed:17391063, ECO:0000269|PubMed:24753243, ECO:0000269|PubMed:34922205, ECO:0000269|PubMed:8528210, ECO:0000269|Ref.18}.; [Isoform 5]: Lacks catalytic activity. {ECO:0000269|PubMed:17391063}.; [Isoform 6]: Lacks catalytic activity. {ECO:0000269|PubMed:17391063}.

NCBI Gene ID

25085

Shipping

Blue Ice

Tyrosine Hydroxylase Ser40 ICC Protocol: Download

Western Blot Protocol: Download

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Anti-Tyrosine Hydroxylase (Ser40) Antibody

SKU: p1580-40

Product Details

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