Anti-TRPML3/Mucolipin-3 Antibody (N268/19)

Name: Anti-TRPML3/Mucolipin-3 Antibody
Brand: NeuroMab
SKU: 75-283
Price: 337.0 USD
Availability: InStock

Our mouse TRPML3/Mucolipin-3 monoclonal antibody from NeuroMab is produced in-house from clone N268/19. It detects human, mouse, and rat TRPML3/Mucolipin-3 and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-283

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

TRPML3/Mucolipin-3

Scientific Background

Mucolipin-3, Transient receptor potential cation channel, mucolipin subfamily, member 3 or TrpML3 is encoded by the gene MCOLN3. It is a member of the small family of the TRPML channels, a subgroup of the large protein family of TRP ion channels and acts as an inwardly rectifying cation channel. TrpML3 is found in the cochlea, olfactory kidney, lung and hair cells and strial cells of the inner ear. The protein localizes to the plasma membrane, endosomes, and lysosomes of cells. Mutations in this gene in mice result in auditory hair cell death and deafness

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N268/19

Isotype

IgG2a

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

MCOLN3

Molecular Weight

64 kDa

Immunogen

Synthetic peptide amino acids 269-285 (CKDWHVSGSIQKNTHYM, extracellular N-terminus) of human TRPML3 (accession number Q8TDD5)

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11000726

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

TRP-ML3; Mucolipin-3; Transient receptor potential channel mucolipin 3; TRPML3

UniProt ID

Q8TDD5
UniProt Summary: Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity. Mediates release of Ca(2+) from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway. Also permeable to Mg(2+), Na(+) and K(+). Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells. Proposed to play a critical role at the cochlear stereocilia ankle-link region during hair-bundle growth. Involved in the regulation of autophagy. Through association with GABARAPL2 may be involved in autophagosome formation possibly providing Ca(2+) for the fusion process. Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events. Possible heteromeric ion channel assemblies with TRPV5 show pharmacological similarity with TRPML3.

NCBI Gene ID

55283

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Anti-TRPML3/Mucolipin-3 Antibody (N268/19)

SKU: 75-283

Product Details

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