Anti-SHP2 (N-terminal region) Antibody (M163)

Name: Anti-SHP2 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SM1631
Price: 292.0 USD
Availability: InStock

Our SHP2 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat SHP2 (N-terminal region) and is Protein A purified. It is great for use in WB.

Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

SKU: SM1631

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

SHP2 (N-terminal region)

Scientific Background

SHP2 (PTP1D, SH-PTP2, or Syp) is a widely expressed protein-tyrosine phosphatase (PTP) that maintains phosphotyrosine homeostasis during growth factor, cytokine, hormone and antigen receptor signaling. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP2 associates with EGF and PDGF growth factor receptors and is activated after stimulation of these receptors. Activation of SHP-2 and its association with Gab1 is critical for sustained ERK activation downstream of both growth factor and cytokine receptors. In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites. Thus, SHP2 is critical for maintaining phosphotyrosine homeostasis in many cell signaling pathways

Format

Protein A Purified

Clonality

Monoclonal

Clone

M163

Isotype

IgG1

Applications

ELISA, WB

Host Species

Mouse

Gene Name

PTPN11

Molecular Weight

Immunogen

Clone (M163) was generated from a recombinant protein containing amino acids in the N-terminal region of human SHP2. This sequence is highly conserved in rat and mouse SHP2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 72 kDa* protein in human A431 and Jurkat cells, and mouse brain. This antibody does not cross-react with SHP1.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

BPTP3; PTP1D; JMML; METCDS; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2; tyrosine-protein phosphatase non-receptor type 11; PTP-2C; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C; SHPTP2; Syp

UniProt ID

Q06124
UniProt Summary: Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification. Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L. Acts as an effector of PDCD1-mediated inhibition of T-cell response: recruited by phosphorylated PDCD1, mediating dephosphorylation of key T-cell receptor (TCR) proximal signaling molecules, leading to TCR signaling inhibition.

NCBI Gene ID

5781

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Anti-SHP2 (N-terminal region) Antibody (M163)

SKU: SM1631

Product Details

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