Anti-SAP49 Antibody (3A1)

Name: Anti-SAP49 Antibody
Brand: PhosphoSolutions
SKU: 1900-SAP
Price: 456.0 USD
Availability: InStock

Our Anti-SAP49 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat SAP49 and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunoflourescence of HeLa cells labeled with anti-SAP49 (Cat. 1900-SAP, red, 1:1000) and anti-vimentin (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Western blot of HeLa lysate showing specific immunolabeling of the ~49 kDa SAP49 protein.

Immunoflourescence of HeLa cells labeled with anti-SAP49 (Cat. 1900-SAP, red, 1:1000) and anti-vimentin (Cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Click on image to zoom

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1900-SAP

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

SAP49

Scientific Background

The spliceosome associated protein of ~49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994). The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006).

Format

Protein G Purified

Clonality

Monoclonal

Clone

3A1

Isotype

IgG2b

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

SF3B4

Molecular Weight

49 kDa

Immunogen

Recombinant full length human SAP49 expressed in and purified from E. Coli.

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2492236

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN₃

Dilution Ranges

WB: 1:2000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~49 kDa SAP49 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

AFD1; Hsh49; U2 snRNP; MGC10828; Pre mRNA splicing factor SF3b 49 kDa subunit; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49; Sf3b4; SF3B4_HUMAN; SF3b49; SF3b50; Spliceosomal protein; Spliceosome associated protein (U2 snRNP; Spliceosome associated protein 49; Spliceosome-associated protein 49; Splicing factor 3b subunit 4 49kDa; Splicing factor 3B subunit 4; Spliceosome associated protein

UniProt ID

Q15427
UniProt Summary: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:12234937, PubMed:27720643, PubMed:32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:12234937, PubMed:32494006). Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937, PubMed:27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932). {ECO:0000269|PubMed:10882114, ECO:0000269|PubMed:12234937, ECO:0000269|PubMed:15146077, ECO:0000269|PubMed:27720643, ECO:0000269|PubMed:32494006, ECO:0000269|PubMed:33509932}.

NCBI Gene ID

10262

Shipping

Blue Ice

SAP49 ICC Protocol: Download

Western Blot Protocol: Download

General References

Champion-Arnaud P, Reed R. (1994) The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes and Development 8(16):1974-83. PMID: 7958871
Terada Y, Yasuda Y. (2006) Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145. Molecular and Cellular Biology 26(21):8149-58. PMID: 16923959

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Anti-SAP49 Antibody (3A1)

SKU: 1900-SAP

Product Details

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