Anti-RGS14 Antibody (N133/35)

Name: Anti-RGS14 Antibody
Brand: NeuroMab
SKU: 75-422
Price: 337.0 USD
Availability: InStock

Our mouse RGS14 monoclonal antibody from NeuroMab is produced in-house from clone N133/35. It detects human, mouse, and rat RGS14 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat hippocampus immunohistochemistry.

$Immunoblot against crude rat brain membrane fractions (RBM) and extracts of COS cells transiently transfected with GFP-tagged rat RGS14 (rRGS14), Flag-tagged human RGS14 (hRGS14), GFP-tagged rat RGS12 (rRGS12) or untagged Kv2.1 plasmid probed with N133/35 (left) or N52A/42 (right) TC supe.$

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-422

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

RGS14

Scientific Background

RGS14 is a member of the RGS family (regulator of G-protein signaling) and is highly expressed in the prefrontal cortex. Hallmark domains of RGS14 include a RGS domain, two Raf-like Ras-binding domains and a GoLoco motif. RGS14 inactivates G protein α subunits. This protein has been implicated in Schizophrenia and Alzheimer’s disease.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N133/35

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Rgs14

Molecular Weight

60 kDa

Immunogen

Fusion protein amino acids 1-544 (full-length) of rat RGS14 (accession number O08773) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2716259

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with RGS12

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

regulator of G-protein signaling 14; RGS14

UniProt ID

O08773
UniProt Summary: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Besides, modulates signal transduction via G protein alpha subunits by functioning as a GDP-dissociation inhibitor (GDI). Has GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not on GNAI2 and G(o)-alpha subunit GNAO1. Has GAP activity on GNAI0, GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.

NCBI Gene ID

114705

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Anti-RGS14 Antibody (N133/35)

SKU: 75-422

Product Details

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