Anti-RBPMS Antibody

Name: Anti-RBPMS Antibody
Brand: PhosphoSolutions
SKU: 1830-RBPMS
Price: 456.0 USD
Availability: InStock

Our Anti-RBPMS primary antibody from PhosphoSolutions is rabbit polyclonal. It detects feline, guinea pig, pig, human, mouse, non-human primate, rabbit, rat, tree shrew, whale, and zebrafish RBPMS and is antigen affinity purified. It is great for use in WB, IHC, ICC.

Immunostaining of mouse retinal ganglion cells showing specific immunolabeling of RBPMS (1:1000) in red. Photo courtesy of Allen Rodriguez, University of California, Los Angeles.

Immunostaining of showing specific immunolabeling of RBPMS. Photo courtesy of Emily Brinker, Auburn University.

Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.

Representative confocal image of a horizontal section of the macaque ganglion cell layer immunolabeled with a pan-RGC marker (RBPMS, Cat. 1830-RBPMS, white) and probed by RNA in situ hybridization for BNC2 (green) and FSTL4 (red) mRNA. Nuclei are counterstained with DAPI. Image from publication CC-BY-4.0.

Whole mount whale retina labelled with RBPMS (red) to label RGCs. Image from publication CC-BY-4.0. PMID: 35185483

Immunostaining of retinas in control, I/R, I/R+DMSO and I/R+VAL groups, stained with DAPI (blue), RBPMS (green) and TUNEL (red). Image from publication CC-BY-4.0. PMID: 36361772

Representative confocal image of macaque GCL immunolabelled for BNC2, FOXP2 and RBPMS (Cat. 1830-RBPMS). Image from publication CC-BY-4.0. PMID: 37880369

Human ganglion cell layer (GCL) labelled for BNC2 and RBPMS (Cat. 1830-RBPMS) with example RGC11 cells in ROIs. ROI 1 and ROI 2 are shown enlarged on the right. Images in b were 2D median filtered before maximal z-projection of the GCL. Image from publication CC-BY-4.0. PMID: 37880369

Immunostaining of mouse retinal ganglion cells showing specific immunolabeling of RBPMS (1:1000) in red. Photo courtesy of Allen Rodriguez, University of California, Los Angeles.

Click on image to zoom

Species Reactivity

Feline, Guinea Pig, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat, Tree Shrew, Whale, Zebrafish

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 1830-RBPMS

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

RBPMS

Scientific Background

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014)

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

RBPMS

Molecular Weight

24 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat RBPMS, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Feline, Guinea Pig, Human, Mouse, Non-Human Primate, Pig, Rabbit, Rat, Tree Shrew, Whale, Zebrafish

Expected Reactivity

Canine, Hamster

Antibody Registry ID

AB_2492225

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Affinity purification via chromatography on an affinity column prepared with the peptide used as antigen.

Buffer

PBS + 0.03% NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:2000
ICC: 1:200-1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~24 kDa RBPMS protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

FLJ32971; Heart and RRM expressed sequence; Hermes; RBP MS; RBP-MS; Rbpms; RBPMS_HUMAN; RNA binding protein gene with multiple splicing; RNA-binding protein with multiple splicing

UniProt ID

Q9WVB0
UniProt Summary: [Isoform A]: RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS) (PubMed:24860013, PubMed:26347403). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets (By similarity). Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state (By similarity). Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats (PubMed:24860013, PubMed:26347403). Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes (By similarity). {ECO:0000250|UniProtKB:A0A8I6G705, ECO:0000269|PubMed:24860013, ECO:0000269|PubMed:26347403}.; [Isoform C]: Acts as a regulator of pre-mRNA alternative splicing (AS) (By similarity). Binds mRNA (PubMed:17099224). Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA (By similarity). Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins (PubMed:17099224). {ECO:0000250|UniProtKB:A0A8I6G705, ECO:0000269|PubMed:17099224}.

NCBI Gene ID

19663

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RBPMS IHF Protocol: Download

Western Blot Protocol: Download

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Anti-RBPMS Antibody

SKU: 1830-RBPMS

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