Anti-PSD-95 Recombinant Guinea Pig Chimeric mAb (K28/43)
Our guinea pig Anti-PSD 95 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone K28/43. It detects chicken, human, mouse, non-human primate, and rat PSD 95 and is purified by affinity chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.
Immunohistochemistry for detection of PSD-95 in adult rat cerebellum using Anti-PSD-95 Recombinant Chimeric Guinea Pig Monoclonal antibody [K28/43] (Cat No. 74-028). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-PSD-95 antibody (Cat No. 74-028) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum.
PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy
Purified by affinity chromatography
1 mg/mL
Recombinant
K28/43
IgG
ELISA, ICC, IHC, IP, WB
Guinea Pig
DLG4 PSD95
95-110 kDa (varies with cell background due to phosphorylation)
Unconjugated
Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli
Human
Chicken, Human, Mouse, Non-Human Primate, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K28/43 with guinea pig IgG heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K28/43 but can be detected using standard anti-guinea pig secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
WB: 1:1000
IHC: 1:500
ICC: 1:500
Unconjugated
No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102
Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Discs large homolog 4; Disks large homolog 4; Disks large homolog 4; Postsynaptic density protein 95; DLG 4; PSD-95; Dlg4; Synapse-associated protein 90; DLG4_HUMAN; SAP-90; FLJ97752; SAP90; FLJ98574; Human post synaptic density protein 95; Post synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; PSD95; SAP 90; SAP-90; SAP90; Synapse associated protein 90; Synapse-associated protein 90; Tax interaction protein 15
P78352
UniProt Summary: Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression. Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins.
1742
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![Immunohistochemistry for detection of PSD-95 in adult rat cerebellum using Anti-PSD-95 Recombinant Chimeric Guinea Pig Monoclonal antibody [K28/43] (Cat No. 74-028). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-PSD-95 antibody (Cat No. 74-028) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum.](http://www.antibodiesinc.com/cdn/shop/files/74-028-ihc-1_120x120.jpg?v=1778860424)
![Western blot detection of PSD-95 using Anti-PSD-95 Recombinant Chimeric Guinea Pig Monoclonal antibody [K28/43] (Cat No. 74-028). 10-20% Tris-Glycine Gel was loaded with rat brain membrane fraction. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-PSD-95 antibody was incubated with sample at a concentration of 1 ug/ml and then detected via chemiluminescence (40 sec) using a Goat Anti-Guinea Pig H+L, HRP conjugated secondary antibody. Anti-PSD-95 recognizes endogenous PSD-95 in the rat brain membrane fraction at the expected molecular weight of ~95-110 kDa.](http://www.antibodiesinc.com/cdn/shop/files/74-028-wb-1_120x120.jpg?v=1778860424)
![Immunohistochemistry for detection of PSD-95 in adult rat cerebellum using Anti-PSD-95 Recombinant Chimeric Guinea Pig Monoclonal antibody [K28/43] (Cat No. 74-028). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-PSD-95 antibody (Cat No. 74-028) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum.](http://www.antibodiesinc.com/cdn/shop/files/74-028-ihc-2_120x120.jpg?v=1778860424)
![Immunohistochemistry for detection of PSD-95 in adult rat cerebellum using Anti-PSD-95 Recombinant Chimeric Guinea Pig Monoclonal antibody [K28/43] (Cat No. 74-028). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-PSD-95 antibody (Cat No. 74-028) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum.](http://www.antibodiesinc.com/cdn/shop/files/74-028-ihc-3_120x120.jpg?v=1778860424)
![Immunohistochemistry for detection of PSD-95 in adult rat cerebellum using Anti-PSD-95 Recombinant Chimeric Guinea Pig Monoclonal antibody [K28/43] (Cat No. 74-028). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-PSD-95 antibody (Cat No. 74-028) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-PSD-95 specifically stains the excitatory synapses in the granular layer of the cerebellum.](http://www.antibodiesinc.com/cdn/shop/files/74-028-ihc-1_1600x.jpg?v=1778860424)
KO Validated
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