Our mouse Pan-MAGUK monoclonal antibody from NeuroMab is produced in-house from clone K28/86. It detects C. elegans, chicken, human, mouse, rat, Xenopus, and zebrafish Pan-MAGUK and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.
Immunoblot against crude C. elegans worm extracts and crude brain membranes from adult rat (RBM) probed with K28/86 TC supe.
Membrane-associated guanylate kinases (MAGUKs) are a large family of scaffold proteins that play essential roles in tissue development, cell-cell communication, cell polarity control, and cellular signal transduction. MAGUKs act as key scaffolds in surface complexes containing receptors, adhesion proteins, and various signaling molecules and play key roles in cell-cell intercommunication. The neuronal synapse is a prime example of this, where the MAGUK proteins play a critical role in organizing both pre and post-synaptic proteins into functional assemblies. Major synaptic MAGUK proteins include PSD-95, SAP102, SAP97, PSD-93, CASK, and MAGIs. Together, they play roles in both synaptic development and in synaptic plasticity.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
K28/86
IgG1
ELISA, ICC, IHC, IP, WB
Mouse
DLG1 DLG2 DLG3 KIAA1232 DLG4 PSD95
95-110 kDa (varies with cell background due to phosphorylation)
Fusion protein amino acids 77-299 of human PSD-95/SAP90 produced recombinantly in E. Coli
Human
C. elegans, Human, Mouse, Rat, Xenopus, Zebrafish
AB_10673115
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
WB: 1:1000
Unconjugated
Exhibits cross-reactivity against recombinant Chapsyn-110, SAP97 and SAP102
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Discs large homolog 4; Disks large homolog 1; Disks large homolog 4; Synapse-associated protein 97; DLG 4; SAP-97; Dlg4; SAP97; DLG4_HUMAN; hDlg Disks large homolog 2; FLJ97752; Channel-associated protein of synapse-110; FLJ98574; Chapsyn-110; Human post synaptic density protein 95; Postsynaptic density protein PSD-93 Disks large homolog 3; Post synaptic density protein 95; Neuroendocrine-DLG; Postsynaptic density protein 95; Synapse-associated protein 102; PSD 95; SAP-102; PSD-95; SAP102; PSD95; XLMR Disks large homolog 4; SAP 90; Postsynaptic density protein 95; SAP-90; PSD-95; SAP90; Synapse-associated protein 90; Synapse associated protein 90; SAP-90; Synapse-associated protein 90; SAP90; Tax interaction protein 15
P78352
UniProt Summary: Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression. Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins.
1742
Shipped on ice packs
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![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with K28/86 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/files/73-029-k28-86-wb-3_120x120.jpg?v=1743796747)
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