Anti-NSE (Neuron specific enolase) Antibody

Name: Anti-NSE (Neuron specific enolase) Antibody
Brand: PhosphoSolutions
SKU: 1520-NSE
Price: 394.0 USD
Availability: InStock

Our Anti-NSE (Neuron specific enolase) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects bovine, chicken, human, mouse, and rat NSE (Neuron specific enolase) and is neat serum. It is great for use in WB, IHC, ICC.

Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (Cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (Cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.

Immunolabeling of HEK 293 cells labeled with anti-neuron specific enolase antibody (Cat. 1520-NSE, red, 1:500) and anti-UCHL1 antibody (Cat. 2060-UCHL1, green, 1:500).

Western blot of rat cortical lysate showing specific immunolabeling of the ~47 kDa NSE protein.

Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (Cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (Cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.

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Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 1520-NSE

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

NSE (Neuron specific enolase)

Scientific Background

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).

Format

Neat Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Gene Name

ENO2

Molecular Weight

47 kDa

Immunogen

Recombinant human NSE expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2492190

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat Serum

Buffer

Neat serum

Dilution Ranges

WB: 1:2000
WB Brain: 1:2000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~47 kDa NSE protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; gamma; Eno 2; ENO2; ENOG; ENOG_HUMAN; Enolase 2 (gamma; neuronal; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Epididymis secretory protein Li 279; Gamma enolase; Gamma-enolase; HEL S 279; Neural enolase; Neuron specific enolase; Neuron specific gamma enolase; Neuron-specific enolase; neuronal enriched enolase; Neurone specific enolase; NSE; Enolase 2

UniProt ID

P09104
UniProt Summary: Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (By similarity). Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). {ECO:0000250|UniProtKB:P06733, ECO:0000250|UniProtKB:P07323}.

NCBI Gene ID

2026

Shipping

Blue Ice

NSE Rat Neurons ICC Protocol: Download

NSE HEK293 Cells ICC Protocol: Download

Western Blot Protocol: Download

General References

MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). European Journal of Biochemistry. 178(2):413-17. PMID: 3208766
Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. Journal of Neurotrauma 23:1201-1210 (2006). PMID: 16928178

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Anti-NSE (Neuron specific enolase) Antibody

SKU: 1520-NSE

Product Details

General References

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