Anti-Npas4 Antibody (N408/79)

Name: Anti-Npas4 Antibody
Brand: NeuroMab
SKU: 75-396
Price: 337.0 USD
Availability: InStock

Our mouse Npas4 monoclonal antibody from NeuroMab is produced in-house from clone N408/79. It detects mouse and rat Npas4 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Hippocampus Immunohistochemistry from adult rats treated with kainic acid.

Immunoblot against crude brain low-speed pellets from untreated adult rats (RBLSP), adult rats treated with kainic acid to induce seizures (RBLSP seiz) and adult rats treated with saline (RBLSP non-seiz) probed with N408/79 (left) and N52A/42 (right) TC supe and with Rabbit anti-c-Fos (middle).

Hippocampus Immunohistochemistry from adult rats treated with kainic acid.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-396

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Npas4

Scientific Background

Neuronal PAS Domain Protein 4 is encoded by the gene NPAS4. NPAS4 is a transcription factor that is involved in a wide range of physiologic and developmental events. NPAS4 is specifically expressed in neurons. No diseases were found to be associated with NPAS4

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N408/79

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Npas4 Nxf

Molecular Weight

90 kDa

Immunogen

Fusion protein amino acids 597-802 (C-terminus) of rat Npas4 (accession number Q8CJH6) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2491085

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Nxf; Neuronal PAS domain-containing protein 4; HLH-PAS transcription factor NXF; Neuronal PAS4; bHLH-PAS type transcription factor NXF

UniProt ID

Q8CJH6
UniProt Summary: Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hippocampus. Plays a key role in the structural and functional plasticity of neurons. Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience. In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons. In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits. The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior. Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2. Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1. Can activate the CME (CNS midline enhancer) element.

NCBI Gene ID

266734

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Anti-Npas4 Antibody (N408/79)

SKU: 75-396

Product Details

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