Our Anti-NGFR/p75 neurotrophin receptor (p75NTR) mouse monoclonal primary antibody detects cat, dog, human, pig, rabbit, and sheep NGFR/p75 neurotrophin receptor (p75NTR), and is IgG. It is validated for use in IHC, IHC-Frozen.
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.
IgG
Monoclonal
ME20.4
IgG1
IHC
Mouse
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Human
Canine, Feline, Human, Pig, Rabbit, Sheep
The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
Liquid
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
PBS pH 7.2-7.6
IHC: 2 µg/mL
ELISA: 1:5000
This antibody is recommended for use in immunohistochemistry, immunofluorescence, and NGF receptor p75 dynamics. Not appropriate for Western Blots or flow cytometry.
FITC Ex: 490 nm, Em: 525 nm
This antibody recognizes p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
P08138
UniProt Summary: Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF. Plays an important role in differentiation and survival of specific neuronal populations during development. Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA. Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake. Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways.
4804
2-8°C (on cold packs)
Bulk Order Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (FITC)
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