Anti-Neurofilament NF-L Antibody (DA2)

Name: Anti-Neurofilament NF-L Antibody
Brand: PhosphoSolutions
SKU: 1452-NFL
Price: 394.0 USD
Availability: InStock

Our Anti-Neurofilament NF-L primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-L and is Protein G purified. It is great for use in WB, IHC, ICC.

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody (Cat. 1452-NFL, green, 1:100) and anti-alpha internexin.

Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (Cat. 1451-NFH, red, 1:25,000) and anti-NFL antibody (Cat. 1452-NFL, green, 1:100).

Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (Cat. 1452-NFL, 1:500, red) and labeling of GFAP (Cat. 621-GFAP, 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.

Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.

Click on image to zoom

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1452-NFL

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neurofilament NF-L

Scientific Background

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about ~68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

Format

Protein G Purified

Clonality

Monoclonal

Clone

DA2

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

NEFL

Molecular Weight

68 kDa

Immunogen

Preparation of porcine NF-L.

Immunogen Species

Pig

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2492161

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified tissue culture supernatant.

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000
ICC: 1:100-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~68 kDa Neurofilament L protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

68 kDa neurofilament protein; 68kDa Neurofilament; 68kDa neurofilament protein; CMT1F; CMT2E; FLJ53642; Light molecular weight neurofilament protein; NEFL; Neurofilament light; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament protein; light chain; Neurofilament subunit NF L; Neurofilament triplet L protein; NF-L; NF68; NFL; NFL_HUMAN

UniProt ID

P02547
UniProt Summary: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). {ECO:0000250|UniProtKB:P08551}.

NCBI Gene ID

4747

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Res 30:47-62. PMID: 10841809
Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. American Journal of Human Genetics 67:37-46. PMID: 1724473

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Anti-Neurofilament NF-L Antibody (DA2)

SKU: 1452-NFL

Product Details

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