Anti-Neurofilament medium polypeptide (NF-M) Antibody

Name: Anti-Neurofilament medium polypeptide (NF-M) Antibody
Brand: Biosensis
SKU: C-1393-50
Price: 337.0 USD
Availability: InStock

Our Anti-Neurofilament medium polypeptide (NF-M) chicken polyclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament medium polypeptide (NF-M), and is IgY preparation. It is validated for use in IF, ICC, IHC, WB.

Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. These cultures were stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red). The Neurofilament Medium (NF-M) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.

Image of rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red, 1:1,000) and co-stained with product M-1802-50, 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP), Mouse mAb (green, 1:500). The blue is DAPI staining of nuclear DNA. Method: Following transcardial perfusion of rat with 4% PFA, brain was post-fixed for 24 hours, cut to 45 uM, and free-floating section was stained. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.

Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. These cultures were stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red). The Neurofilament Medium (NF-M) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.

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Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Applications

ICC, IF, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1393-50

Volume/Size: 50 µL

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Neurofilament medium polypeptide (NF-M)

Scientific Background

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70 kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)

Format

IgY preparation

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IF, IHC, WB

Host Species

Chicken

Molecular Weight

~145 kDa for rodent and ~160 kDa for human and cow

Immunogen

This antibody has been made against a recombinant fusion protein containing the extreme C-terminus of human NF-M (amino acids 708-877) expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:2000
ICC: 1:2000

Conjugate

Unconjugated

Specificity

Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

150 kDa Neurofilament Medium; NF-M; 160 kDa neurofilament protein; NFM; Medium polypeptide 150kDa; Neurofilament medium polypeptide; NEF3; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament 3; Neurofilament 3 medium; Neurofilament triplet M protein; Neurofilament medium polypeptide; Nefm; Neurofilament protein M; Nef3; Neurofilament triplet M protein; Neurofilament-3 (150 kD medium; NF-M; NFM

UniProt ID

P07197
UniProt Summary: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

NCBI Gene ID

4741

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25°C (ambient)

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Anti-Neurofilament medium polypeptide (NF-M) Antibody

SKU: C-1393-50

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