Anti-Nav1.1 Na+ Channel Recombinant Chicken Chimeric mAb (K74/71)

Name: Anti-Nav1.1 Na+ Channel Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-023
Price: 481.0 USD
Availability: InStock

Our chicken Anti-Nav1.1 Na+ channel recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone K74/71. It detects human, mouse, non-human primate, and rat Nav1.1 Na+ channel and is purified by affinity chromatography. Works in WB, IHC, ICC, IP, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by anti-Nav1.1 (SCN1A) antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

$Western blotting of rat brain membrane with anti-Nav1.1 (SCN1A) recombinant chicken mAb (Cat. 78-023) antibody. The chicken mAb was detected with anti-chicken-HRP (Cat. 80-1000-HRP). Aves Labs Recombinant Chicken anti-Nav1.1 mAb recognizes endogenous Nav1.1 in the rat brain membrane fraction at ~220 kDa.$

Representative section of formalin fixed, paraffin-embedded rat brain hippocampal CA2/CA3 region showing cells stained by anti-Nav1.1 (SCN1A) antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by anti-Nav1.1 (SCN1A) antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-023

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Nav1.1 Na+ channel

Scientific Background

Nav1.1 Na+ channel (sodium channel, voltage-gated, type I, alpha subunit/ SCN1A) is a member of voltage-gated sodium ion channel subunit family. It is encoded by gene Scn1a in human.The channel switches between open and close conformation in response to the voltage difference accross the membrane. Nav1.1 Na+ channel is a sodium selective channel that maintains Na+ homeostasis by allowing Na+ ions to pass in accordance of their electrochemical gradient. The protein plays an important role in the release of neurotransmittors from the neurons. Therefore, Nav1.1 Na+ channel is involved in the perception of mechanical pain due to the activation of sematosensory neurons without the involvement of inflammation. Mutation of the gene encoding for Nav1.1 Na+ channel is one of the main cause of epilepsy and febrile seizures.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

K74/71

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

Scn1a

Molecular Weight

220 kDa

Immunogen

Fusion protein amino acids 1929-2009 (cytoplasmic C-terminus) of rat Nav1.1 (accession number P04774) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K74/71 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K74/71 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity with Nav1.2, Nav1.3 and Nav1.6

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

sodium channel protein; Sodium channel protein type 1 subunit alpha; brain I subunit alpha; Sodium channel protein brain I subunit alpha; Sodium channel protein type I subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.1

UniProt ID

P04774
UniProt Summary: Pore-forming subunit of Nav1.1, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues. By regulating the excitability of neurons, ensures that they respond appropriately to synaptic inputs, maintaining the balance between excitation and inhibition in brain neural circuits. Nav1.1 plays a role in controlling the excitability and action potential propagation from somatosensory neurons, thereby contributing to the sensory perception of mechanically-induced pain.

NCBI Gene ID

81574

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Anti-Nav1.1 Na+ Channel Recombinant Chicken Chimeric mAb (K74/71)

SKU: 78-023

Product Details

Bulk Order Anti-Nav1.1 Na+ Channel Recombinant Chicken Chimeric mAb

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