Anti-MANF Antibody

Name: Anti-MANF Antibody
Brand: Biosensis
SKU: M-1673-100
Price: 467.0 USD
Availability: InStock

Our Anti-Mesencephalic astrocyte-derived neurotrophic factor (MANF) mouse monoclonal primary antibody detects human Mesencephalic astrocyte-derived neurotrophic factor (MANF), and is IgG. It is validated for use in WB.

Western Blot testing of anti-MANF monoclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85 cell line.

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Species Reactivity

Human

Applications

Host Species

Mouse

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Data Sheet

SKU: M-1673-100

Volume/Size: 100 µg

Variant

Price:

$467.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Mesencephalic astrocyte-derived neurotrophic factor (MANF)

Scientific Background

MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Format

IgG

Clonality

Monoclonal

Isotype

IgG1

Applications

Host Species

Mouse

Immunogen

Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.

Immunogen Species

Human

Species Reactivity

Human

Storage

Store at -20°C or -70°C upon receipt. After opening maintain undiluted in smaller aliquots at -20°C or -70°C for up to 6 months. Avoid multiple freeze-thaw cycles.

Physical State

Liquid

Production Notes

Purified with Protein G affinity chromatography

Buffer

PBS pH 7.4, with 0.1% sodium azide

Dilution Ranges

WB: 0.5-2 µg/mL
IHC: 1-10 µg/mL
ICC: 1-10 µg/mL
ELISA: 0.1-0.2 µg/mL (indirect)

Conjugate

Unconjugated

Specificity

MANF (Mesencephalic astrocyte-derived neurotrophic factor).

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

ARMET; arginine-rich; mutated in early stage tumors; ARP

UniProt ID

P55145
UniProt Summary: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions. Stabilizes HSPA5/BiP in its substrate-bound ADP state, which facilitates HSPA5/BiP incorporation into chaperone-client complexes during endoplasmic reticulum stress, its interaction with HSPA5/BiP inhibits ATP binding to HSPA5/BiP and subsequent nucleotide exchange. As a result acts as a repressor of the unfolded protein response (UPR) pathway. Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia. Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. Essential for embryonic lung development. Required for the correct postnatal temporal and structural development of splenic white pulp. Required for the repair-associated myeloid response in skeletal muscle, acts as a regulator of phenotypic transition towards prorepair macrophages in response to muscle injury and as a result limits excessive proinflammatory signaling. Represses RELA expression and therefore NF-kB signaling in the myocardium, as a result limits macrophage infiltration of injured tissue and M1 macrophage differentiation in response to myocardial injury. Required for endochondral ossification in long bones and the skull during postnatal development.

NCBI Gene ID

7873

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25°C (ambient)

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Anti-MANF Antibody

SKU: M-1673-100

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