Anti-Kv3.3 Potassium Channel Antibody (N375/67)

Name: Anti-Kv3.3 Potassium Channel Antibody
Brand: NeuroMab
SKU: 75-354
Price: 337.0 USD
Availability: InStock

Our mouse Kv3.3 potassium channel monoclonal antibody from NeuroMab is produced in-house from clone N375/67. It detects human, mouse, and rat Kv3.3 potassium channel and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat hippocampus immunohistochemistry.

$Immunoblot against adult rat brain membrane (RBM) fractions and extracts of COS cells transiently transfected with untagged Kv3.3a, Kv3.3b or Kv2.1 plasmid probed with N375/67 (left) or N52A/42 (right) TC supe.$

Adult rat hippocampus immunohistochemistry.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-354

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kv3.3 potassium channel

Scientific Background

Potassium voltage-gated channel subfamily C member 3 or Kv3.3 potassium channel, is a member of the potassium channel, voltage-gated, shaker-related subfamily and the Shaw subfamily (the family includes 4 members Kv3.1-Kv3.4). Note that this protein has several splice variants and that antibody N375/67 recognizes both Kv3.3b and Kv3.3a. Kv3.3 is primarily expressed in brain. Kv3.3 is highly expressed in purkinje cells and deep cerebellar nuclei in the cerebellum. Kv3.3a is also expressed in brain, but not found in the cerebellum. Kv3.3 is a membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Diseases associated with the gene for this protein (Kcnc3) include Spinocerebellar Ataxia 13 and Autosomal recessive ataxic cerebral palsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N375/67

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnc3

Molecular Weight

80-100 kDa (depending on isoform)

Immunogen

Fusion protein amino acids 628-728 (cytoplasmic C-terminus) of mouse Kv3.3b (accession number Q63959) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315872

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Kv3.4 or Kv3.2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

KShIIID; Potassium voltage-gated channel subfamily C member 3; Kv3.3; voltage-gated potassium channel subunit Kv3.3

UniProt ID

Q63959
UniProt Summary: Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient. The channel displays rapid activation and inactivation kinetics. It plays a role in the regulation of the frequency, shape and duration of action potentials in Purkinje cells. Required for normal survival of cerebellar neurons, probably via its role in regulating the duration and frequency of action potentials that in turn regulate the activity of voltage-gated Ca(2+) channels and cellular Ca(2+) homeostasis. Required for normal motor function. Plays a role in the reorganization of the cortical actin cytoskeleton and the formation of actin veil structures in neuronal growth cones via its interaction with HAX1 and the Arp2/3 complex.

NCBI Gene ID

16504

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Anti-Kv3.3 Potassium Channel Antibody (N375/67)

SKU: 75-354

Product Details

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