Anti-Iduna/RNF146 Antibody (N201/35)

Name: Anti-Iduna/RNF146 Antibody
Brand: NeuroMab
SKU: 75-233
Price: 337.0 USD
Availability: InStock

Our mouse Iduna/RNF146 monoclonal antibody from NeuroMab is produced in-house from clone N201/35. It detects human, mouse, and rat Iduna/RNF146 and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Adult rat brain membrane immunoblot.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-233

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Iduna/RNF146

Scientific Background

E3 ubiquitin-protein ligase RNF146, RING finger protein 146 or Iduna is encoded by the gene RNF146. Iduna is a ubiquitin protein ligase that is involved in ubiquitination and degradation. It is thought to be involved with diverse processes including DNA repair, activation of the Wnt signaling pathway, protection from DNA damage induced cell death. Iduna is found in many tissues including brain, heart, spleen, kidney and liver.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N201/35

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

Rnf146

Molecular Weight

40 kDa

Immunogen

Fusion protein amino acids 1-359 (full-length) of mouse Iduna (accession number Q9CZW6) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10672248

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

2610509H23Rik; E3 ubiquitin-protein ligase RNF146; EC 2.3.2.27; RING-type E3 ubiquitin transferase RNF146; Iduna; RING finger protein 146

UniProt ID

Q9CZW6
UniProt Summary: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.

NCBI Gene ID

68031

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Anti-Iduna/RNF146 Antibody (N201/35)

SKU: 75-233

Product Details

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