Anti-GluN1/NR1 Glutamate Receptor Recombinant Chicken Chimeric mAb FL650 Conjugate (N308/48)
Our chicken Anti-GluN1/NR1 glutamate receptor FL650 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N308/48. It detects human, mouse, and rat GluN1/NR1 glutamate receptor and is purified by affinity chromatography. Works in IHC, ICC.
Anti-GluN1/NR1 Glutamate Receptor Recombinant Chicken Chimeric mAb FL650 Conjugate
Glutamate receptor 1, NMDA 1, or GluN1/NR1 also known as subunit 1 of the N-methyl-D-aspartate (NMDA) receptor. It is a member of the glutamate receptor channel superfamily and is a heteromeric glutamate-gated calcium ion channel. GluN1 is essential for synaptic function in the brain. GluN1 is expressed throughout the brain in cerebellum, thalamus, olfactory bulb and cortex in the membrane at postsynaptic density. GluN1 plays a key role in synaptogenesis, synaptic plasticity, memory acquisition and learning. Mutations in the GluN1 gene are associated with intellectual disability and cortical visual impairment and movement disorders.
Purified by affinity chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Recombinant
N308/48
IgY
ICC, IHC
Chicken
Grin1 Nmdar1
105 kDa
FL650 Ex: 655 nm, Em: 676 nm
Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1 (accession number P35439) produced recombinantly in E. Coli
Rat
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N308/48 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N308/48 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.
1X PBS, 0.05% Sodium Azide pH 7.4
IHC: 1:500-1:1000
ICC: 1:250-1:500
650
No cross-reactivity reported
Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
P35439
UniProt Summary: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators.
Trial Size