Anti-Galectin-3 (Gal-3) Antibody (5C21)

Name: Anti-Galectin-3 (Gal-3) Antibody
Brand: Biosensis
SKU: M-1574-100
Price: 337.0 USD
Availability: InStock

Our Anti-Galectin-3 (Gal-3) mouse monoclonal primary antibody detects human, mouse, other mammals (predicted), and rat Galectin-3 (Gal-3), and is affinity-purified. It is validated for use in ICC, WB.

Image shows rat brain neural cultures stained with Mouse Monoclonal Antibody to Galectin-3 M-1574-100 (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.

Left: Analysis of galectin-3 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with mouse antibody to galectin-3 (red, 1:1,000), and co-stained with chicken antibody to MAP2 (C-1382-50, green, 1:10,000). Blue: Hoechst nuclear stain. The galectin-3 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while MAP2-antibody labels dendrites and perikarya of mature neurons. Right: Western blot analysis of tissue and cell lysates using mouse antibody to galectin-3 (green, 1:2,000): [1] protein standard, [2] mouse brain lysate; [3] - [7] rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at 30 kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with an antibody to HSP60 (red, lanes 2-8).

Image shows rat brain neural cultures stained with Mouse Monoclonal Antibody to Galectin-3 M-1574-100 (green) and DNA (blue). Staining can be seen in several types of glia and lymphocytic cells, including these cells which have the morphology of microglia. Surrounding cells reveal no Galectin-3 staining.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1574-100

Volume/Size: 100 µg

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Galectin-3 (Gal-3)

Scientific Background

Galectin 3 is a lectin with carbohydrate recognition domains (CRD) which bind -galactoside. It is a multifunctional protein expressed both on the cell surface, cytoplasm and nucleus and appears to have roles in specific carbohydrate binding and in the regulation of mRNA splicing.

Format

Affinity-purified

Clonality

Monoclonal

Clone

5C21

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Immunogen

Full length recombinant Galectin-3 expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:2000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Human; mouse; rat; predicted to react with other species due to sequence homology;.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

CBP35; Macrophage galactose-specific lectin; GALBP; MAC2; GALIG; galactoside-binding protein; L31; LGALS2; Galectin 3; Gal 3; galectin-3; 35 kDa lectin; Carbohydrate-binding protein 35; IgE-binding protein; CBP 35; MAC-2 antigen; Galactose-specific lectin 3; advanced glycation end-product receptor 3; L-31; Laminin-binding protein; Lectin L-29; lectin; LGALS3,; galactoside-binding; soluble

UniProt ID

P17931
UniProt Summary: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

NCBI Gene ID

3958

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25°C (ambient)

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Anti-Galectin-3 (Gal-3) Antibody (5C21)

SKU: M-1574-100

Product Details

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