Anti-Dicer Antibody (N167/7)

Name: Anti-Dicer Antibody
Brand: NeuroMab
SKU: 75-196
Price: 337.0 USD
Availability: InStock

Our mouse Dicer monoclonal antibody from NeuroMab is produced in-house from clone N167/7. It detects human, mouse, and rat Dicer and is purified by Protein A chromatography. KO validated. Works in WB, ICC, ELISA.

Immunoblot versus adult rat brain membranes (RBM) and extracts of embryonic stem (ES) cells from wild-type (WT) and Dicer knockout (KO) mice probed with N167/7 (left) and N52A/42 (right, loading control) TC supe. Mouse ES cell samples courtesy of Chryssa Kanellopoulou and David Livingston, Harvard.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-196

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Dicer

Scientific Background

Endoribonuclease Dicer or Dicer 1, Ribonuclease III is encoded by the gene DICER1. DICER1 acts as a double stranded (ds) ribonuclease and plays a role in short post transcriptional gene silencing. DICER1 cleaves full length dsRNA and miRNA into smaller moleclues 21-23 nucleotides in length, called siRNAs and microRNAs. Dicer1 is expressed in a variety of tissues, including brain. Diseases associated with this gene include Pleuropulmonary Blastoma, Glow Syndrome and Wilms Tumor.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N167/7

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

Dicer1 Dicer Mdcr

Molecular Weight

215 kDa

Immunogen

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer (accession number Q8R418) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2093085

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.124

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

DCR1; Endoribonuclease Dicer; Dicer1e; EC 3.1.26.3; HERNA; Double-strand-specific ribonuclease mDCR-1; K12H4.8-LIKE; MNG1; RMSE2; Dicer1; Dcr-1 homolog; dicer 1; ribonuclease type III; helicase MOI; helicase with RNAse motif

UniProt ID

Q8R418
UniProt Summary: Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

NCBI Gene ID

23405

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Anti-Dicer Antibody (N167/7)

SKU: 75-196

Product Details

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