Anti-Connexin-43 (C-terminal region) Antibody (M496)

Name: Anti-Connexin-43 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM4961
Price: 292.0 USD
Availability: InStock

Our Connexin-43 (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Connexin-43 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of connexin-43 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-connexin-43 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: CM4961

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Connexin-43 (C-terminal region)

Scientific Background

Connexin-43 (Cx43, CXA1, Gap Junction α1) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels that cluster together to form gap junctions. Gap junction communication is critical for cell to cell communication during development and regulation of cell growth. Phosphorylation of connexin-43 is important in regulating both the assembly and the function of gap junctions. PKC phosphorylates Ser-368 in connexin-43 after activation of cells with phorbol esters. This phosphorylation event decreases cell-to-cell communication. In addition, the tyrosine kinase, c-Src, can interact with and phosphorylate Tyr-265 in connexin-43 leading to inhibition of gap junction communication. Thus, connexin-43 phosphorylation may be an important mode for regulating gap junctional communication.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M496

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

GJA1

Molecular Weight

Immunogen

Clone M496 was generated from a peptide fragment in the C-terminal region of human connexin-43. This sequence is highly conserved in mouse and rat connexin-43.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 43 kDa* protein corresponding to the apparent molecular mass of connexin-43 on SDS-PAGE immunoblots of mouse brain. The antibody is also useful for immunofluorescent labeling of gap junctions in cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Connexin 43; Gap junction alpha-1 43 GJA1 CXA1; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43; Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3

UniProt ID

P17302
UniProt Summary: Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (Probable). Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in the conductive system of ventricular myocardium and heart morphogenesis. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Involved in intercellular innate immune signaling. Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response.

NCBI Gene ID

2697

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Anti-Connexin-43 (C-terminal region) Antibody (M496)

SKU: CM4961

Product Details

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