Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody

Name: Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody
Brand: PhosphoSolutions
SKU: 325-CNP
Price: 394.0 USD
Availability: InStock

Our Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, rabbit, rat, and sheep CNP (2,3-cyclic nucleotide-3-phosphodiesterase) and is neat serum. It is great for use in WB, IHC.

Immunochemical staining of saline treated mouse cortex cryosections showing specific labeling of CNP (Cat. 325-CNP, green, 1:500). The blue is staining DNA. The bisected longitudinal mouse brain was fixed for 18h in 4% paraformaldehyde, washed, and cryoprotected in 30% sucrose overnight. The cryosections were antigen retrieved in citrate buffer, blocked in 20% normal goat serum for 1 hr. Photo courtesy of Robert Wine.

Western blot of rat brain lysate showing the specific immunolabeling of the ~46 kDa CNP protein.

Immunolabelling of the CA3 subfield of mouse hippocampus labeling CNP (Cat. 325-CNP, green, 1:500) and β-III tubulin (Cat. 2020-TUB, 1:1000, red). The blue is DAPI staining DNA. Original magnification is 40X. Photo courtesy Rob Wine.

Immunochemical staining of saline treated mouse cortex cryosections showing specific labeling of CNP (Cat. 325-CNP, green, 1:500). The blue is staining DNA. The bisected longitudinal mouse brain was fixed for 18h in 4% paraformaldehyde, washed, and cryoprotected in 30% sucrose overnight. The cryosections were antigen retrieved in citrate buffer, blocked in 20% normal goat serum for 1 hr. Photo courtesy of Robert Wine.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat, Sheep

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: 325-CNP

Volume: 50 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CNP (2,3-cyclic nucleotide-3-phosphodiesterase)

Scientific Background

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Format

Neat serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Rabbit

Gene Name

CNP

Molecular Weight

46 kDa

Immunogen

Endogenous rabbit 2,3 cyclic nucleotide-3-phospho-diesterase.

Immunogen Species

Rabbit

Species Reactivity

Human, Mouse, Rat, Sheep

Antibody Registry ID

AB_2492063

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat serum

Buffer

Neat serum

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~46 kDa CNP protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1; CNPase

UniProt ID

P13233
UniProt Summary: Myelin-associated enzyme that catalyzes the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. In the mitochondria, regulates the functioning of the mitochondrial permeability transition pore (mPTP), and thus is involved in the mechanisms of cell death, both apoptosis and necrosis. Acts as an antiviral factor by suppressing the assembly of SARS-CoV-2 virions (PubMed:38117830). {ECO:0000250|UniProtKB:P06623, ECO:0000250|UniProtKB:P16330, ECO:0000269|PubMed:38117830}.; Acts as an antiviral factor by suppressing the assembly of HIV-1 virions through direct interaction with GAG. {ECO:0000269|PubMed:38167610}.

NCBI Gene ID

25275

Shipping

Blue Ice

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Anti-CNP (2,3-cyclic nucleotide-3-phosphodiesterase) Antibody

SKU: 325-CNP

Product Details

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