Anti-Cdc42 Antibody (M152)

Name: Anti-Cdc42 Antibody
Brand: PhosphoSolutions
SKU: CM1521
Price: 292.0 USD
Availability: InStock

Our Cdc42 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Cdc42 and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).

Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (CM1521) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.

Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: CM1521

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Cdc42

Scientific Background

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M152

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

CDC42

Molecular Weight

Immunogen

Clone (M152) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 21 kDa* protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

cell division control protein 42 homolog; CDC-42; cell division cycle 42 homolog; G25K

UniProt ID

Q8CFN2
UniProt Summary: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups. Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation.

NCBI Gene ID

64465

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Anti-Cdc42 Antibody (M152)

SKU: CM1521

Product Details

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