Anti-CamKII subunit alpha, Thr253 Antibody

Name: Anti-CamKII subunit alpha, Thr253 Antibody
Brand: Biosensis
SKU: R-1706-100
Price: 337.0 USD
Availability: InStock

Our Anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) rabbit polyclonal primary antibody detects rat Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253), and is whole serum. It is validated for use in WB.

Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.

Western blots of wild-type CaMKII showing auto-phosphorylation over 4 min at 10 µM ATP, 30°C and probed with antibodies to α-CaMKII, phospho-Thr286-CaMKII (anti-pT286) and phospho-Thr253-CaMKII (anti-pT253). The arrow-heads indicate the electrophoretic mobility of CaMKII phosphorylated at Thr253. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.

Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.

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Species Reactivity

Rat

Applications

Host Species

Rabbit

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Data Sheet

SKU: R-1706-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253)

Scientific Background

Calcium/calmodulin-stimulated protein kinase II (CaMKII) is composed of four different chains (alpha, beta, gamma, and delta) and is abundantly expressed in neurons. CaMKII is involved in regulating many aspects of neuronal function, including neurotransmitter synthesis and release, modulation of ion channel activity and cellular transport. The enzymatic function of CaMKII is regulated by its multiple phosphorylation sites and targeting to sub-cellular locations through interactions with protein binding partners. Phosphorylation of Thr253 has been identified in vivo and found to alter the interaction of CaMKII with binding partners, but not change its enzymatic activity. Thus, phosphorylation of Thr253 is suggested to modulate functional responses based on its binding partner and subsequently its sub-cellular localization.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

Host Species

Rabbit

Immunogen

A synthetic peptide (NKmLpTINPSC) corresponding to the sequence around Thr253 (AA 249-257) in alpha-CaMKII was synthesized, purified to 95% purity by HPLC, analyzed by mass spectroscopy and coupled to diphtheria toxoid.

Immunogen Species

Rat

Species Reactivity

Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:200-1:1000

Conjugate

Unconjugated

Specificity

Rat. Predicted from gene analysis to react with human and mouse alpha-CaMKII.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase II delta; Calcium/calmodulin dependent protein kinase II gamma; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha; CaM kinase II; CaM kinase II beta; CaM kinase II delta; CaM kinase II gamma; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2; Camk2a; CAMK2B; CAMK2D; CAMK2G; CAMKA; KCC2A_HUMAN

UniProt ID

P11275
UniProt Summary: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway. In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.

NCBI Gene ID

25400

Shipping

25°C (ambient)

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Anti-CamKII subunit alpha, Thr253 Antibody

SKU: R-1706-100

Product Details

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