Anti-Ankyrin-B Antibody (N105/13)

Name: Anti-Ankyrin-B Antibody
Brand: NeuroMab
SKU: 75-144
Price: 394.0 USD
Availability: InStock

Our mouse Ankyrin-B monoclonal antibody from NeuroMab is produced in-house from clone N105/13. It detects bovine, guinea pig, human, mouse, and rat Ankyrin-B and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Adult rat hippocampus immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Bovine, Guinea Pig, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-144

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ankyrin-B

Scientific Background

Ankyrin-2 or -Ankyrin-B is encoded by the gene ANK2. Ankyrin B is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin B is expressed in brain and muscle. Mutations in this gene cause long QT syndrome 4 and cardiac arrhythmia syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N105/13

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

ANK2 ANKB

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 203-496 of human Ankyrin-B (accession number Q01484) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Bovine, Guinea Pig, Human, Mouse, Rat

Antibody Registry ID

AB_10673094

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.70

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:500
IP: 3 ug

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ANK-2; Ankyrin-2; brank-2; Ankyrin-B; ankyrin 2; Brain ankyrin; neuronal; Non-erythroid ankyrin; ankyrin B; ankyrin; brain; nonerythrocytic

UniProt ID

Q01484
UniProt Summary: Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate. In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration.

NCBI Gene ID

287

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Anti-Ankyrin-B Antibody (N105/13)

SKU: 75-144

Product Details

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