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Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long, untagged Kirrel2, Kirrel1 or Kv2.1 plasmid and probed with N321C/49 (left) and N52A/42 (right) TC supe.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long or untagged Kv2.1 plasmids and probed with N320/48 (left) and Mouse anti-Flag (right).
Western Blot of endometrial cancer cell lines RL95-2 (lane 1) and KLE (lane 2) showing specific immunolabeling of KLK-L1/KLK4.
Western Blot of BT-474 cell lysate showing specific immunolabeling of Kallikrein 5.
Western Blot of cancer cell lines LnNCap (lane 1) and BT-474 (lane 2) showing specific immunolabeling of KLK-L3.
Western Blot of LNCaP cell lysate showing specific immunolabeling of Kallikrein 13 (KLK-L4).
Immunoprecipitation of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.Western blot of T47D cells (left) and HeLa cells (right) showing specific immunolabeling of the ~80 kDa Ku protein at a dilution of 1:500.
Immunoprecipitation showing detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.Western Blot of 50 ug lysates of A431 (lane 1) and AR42J (lane 2) cells showing specific immunolabeling of Ku80.

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