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Adult rat brain membrane immunohistochemistry.Adult rat brain membrane immunoblot.
Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.Immunoblot versus membranes from postnatal day 8 wild-type (wt) and ADAM22-knockout (ko) mice and probed with N46/30 (left) and N57/2 (right). Data courtesy of Dies Meijer, Erasmus University Rotterdam.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and human cell immunoblot: lysates of RBM and fibroblasts from unaffected (control/normal) and disease-affected (patient/Pex5-mutant) humans probed with N444/63 TC supe (left) and rabbit polyclonal (right).  Human fibroblast samples courtesy of Ning Huang and Joseph Hacia (USC) and rabbit polyclonal courtesy of Nancy Braverman (McGill).
Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.

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