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Adult rat brain membrane immunoblot.Immunoblot versus membranes from postnatal day 8 wild-type (wt) and ADAM22-knockout (ko) mice and probed with N46/30 (left) and N57/2 (right). Data courtesy of Dies Meijer, Erasmus University Rotterdam.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and human cell immunoblot: lysates of RBM and fibroblasts from unaffected (control/normal) and disease-affected (patient/Pex5-mutant) humans probed with N444/63 TC supe (left) and rabbit polyclonal (right).  Human fibroblast samples courtesy of Ning Huang and Joseph Hacia (USC) and rabbit polyclonal courtesy of Nancy Braverman (McGill).
Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.Immunohistochemistry (IHC) staining of Rhesus macaque brain for astrocyte marker Aldh1L1 using Anti-Aldh1L1 Antibody 75-140 (clone N103/39, 1:3000). Tissue was perfusion fixed with 4% paraformaldehyde and 0.1% glutaraldehyde, then drop-fixed in 4% paraformaldehyde for 24 h. Sections were cut in 60 μm thick coronal sections using a vibrating microtome. Image courtesy of Adriana Galvan (Emory University). Additional experimental details can be found at: pubmed.ncbi.nlm.nih.gov/35202614.
Chicken anti-SNCA (green) and TH (red) double immunostaining in Parkinson's Disease patient’s substantia nigra section. Arrows indicate SNCA positive, degenerating dopamine neurons. Arrowheads indicate SNCA negative, healthy dopamine neurons. Bar = 50 um for A-D. (Image courtesy of Dr. Curt Freed’s lab, University of Colorado Anschutz Medical Campus.)Rat brain lysate was probed with no primary antibody as a control (Lane 1) or with a 1:2,500 dilution of Chicken anti-SNCA IgY (Lane 2).

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