Anti-Pan-Neurofascin (Extracellular) Antibody (A12/18)

Name: Anti-Pan-Neurofascin (Extracellular) Antibody
Brand: NeuroMab
SKU: 75-172
Price: 394.0 USD
Availability: InStock

Our mouse Pan-Neurofascin (extracellular) monoclonal antibody from NeuroMab is produced in-house from clone A12/18. It detects human, non-human primate, and rat Pan-Neurofascin (extracellular) and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

$Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.$

Neurons transfected with TfR-SEP and mScarlet-NavII-III, fixed at DIV10, and stained with anti-neurofascin (NF) antibody (clone A12/18) and anti-mScarlet antibody. Intense staining of both mScarlet-NavII-III and NF defines the AIS. 18/20 transfected neurons (from 3 cultures) showed colocalization with endogenous NF. Image from publication CC-BY-4.0. PMID: 35978188

Biotinylated proteins colocalize with endogenous protein signals in the axon initial segment, soma, and somatodendrites. The proximity labeling was directed by anti-NFASC (Cat. 75-172, 1:1000), anti-NeuN, and anti-MAP2 antibodies individually in DIV14 rat cortical neurons with one minute of reaction time. Image from publication CC-BY-4.0. PMID:38081810

Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Human, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-172

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pan-Neurofascin (extracellular)

Scientific Background

Neurofascin is encoded by the gene NFASC. Two major isoforms of NeuroFascin are expressed as a result of alternative splicing, NF186 and NF155. These proteins are expressed in the central nervous system. In brain, NF186 is expressed at Nodes of Ranvier andNF155 is expressed in unmyelinated axons. Neuofascin plays a role in cell adhesion and is thought to be involved in processes such as neurite extension, synaptogenesis and myelination. Diseases associated with this gene include Demyelinating Polyneuropathy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

A12/18

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Nfasc

Molecular Weight

155, 186 kDa

Immunogen

Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin (accession number P97685) by: -binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186);

Immunogen Species

Rat

Species Reactivity

Human, Non-Human Primate, Rat

Antibody Registry ID

AB_2282826

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.100

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Neurofascin NF-155 NF-186

UniProt ID

P97685

Shipping

Shipped on ice packs

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Anti-Pan-Neurofascin (Extracellular) Antibody (A12/18)

SKU: 75-172

Product Details

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